Special considerations for studies of extracellular vesicles from parasitic helminths: A community-led roadmap to increase rigour and reproducibility

White, Ruby and Sotillo, Javier and Ancarola, Maria Eugenia and Borup, Anne and Boysen, Anders Toftegaard and Brindley, Paul J. and Buzás, Edit I. and Cavallero, Serena and Chaiyadet, Sujittra and Chalmers, Iain W. and Cucher, Marcela A. and Dagenais, Maude and Davis, Chelsea N. and Devaney, Eileen and Duque-Correa, Maria A. and Eichenberger, Ramon Marc and Fontenla, Santiago and Gasan, Thomas A. and Hokke, Cornelis H. and Kosanovic, Maja and Kuipers, Marije E. and Laha, Thewarach and Loukas, Alex and Maizels, Rick M. and Marcilla, Antonio and Mazanec, Hynek and Morphew, Russell M. and Neophytou, Kyriaki and Nguyen, Linh Thuy and Nolte-‘t Hoen, Esther and Povelones, Michael and Robinson, Mark W. and Rojas, Alicia and Schabussova, Irma and Smits, Hermelijn H. and Sungpradit, Sivapong and Tritten, Lucienne and Whitehead, Bradley and Zakeri, Amin and Nejsum, Peter and Buck, Amy H. and Hoffmann, Karl F. (2023) Special considerations for studies of extracellular vesicles from parasitic helminths: A community-led roadmap to increase rigour and reproducibility. JOURNAL OF EXTRACELLULAR VESICLES, 12 (1). No.-12298. ISSN 2001-3078

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Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found in the phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function and physiology. Emerging evidence supports a role for helminth-derived EVs in these processes and highlights EVs as an important participant in cross-phylum communication. While the mammalian EV field is guided by a community-agreed framework for studying EVs derived from model organisms or cell systems [e.g., Minimal Information for Studies of Extracellular Vesicles (MISEV)], the helminth community requires a supplementary set of principles due to the additional challenges that accompany working with such divergent organisms. These challenges include, but are not limited to, generating sufficient quantities of EVs for descriptive or functional studies, defining pan-helminth EV markers, genetically modifying these organisms, and identifying rigorous methodologies for in vitro and in vivo studies. Here, we outline best practices for those investigating the biology of helminth-derived EVs to complement the MISEV guidelines. We summarise community-agreed standards for studying EVs derived from this broad set of non-model organisms, raise awareness of issues associated with helminth EVs and provide future perspectives for how progress in the field will be achieved.

Item Type: Article
Additional Information: The University of Edinburgh, Institute of Immunology and Infection Research, School of Biological Sciences, Edinburgh, United Kingdom Instituto de Salud Carlos III, National Center for Microbiology, Madrid, Majadahonda, Spain Department of Microbiology, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina Institute of Research on Microbiology and Medical Parasitology (IMPaM, UBA-CONICET), University of Buenos Aires, Buenos Aires, Argentina Department of Clinical Medicine, Aarhus University, Aarhus, Denmark George Washington University, Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, Washington, DC, United States ELKH-SE Immune Proteogenomics Extracellular Vesicle Research Group, Budapest, Hungary HCEMM-SU Extracellular Vesicle Research Group, Budapest, Hungary Department of Public health and infectious diseases, Sapienza University of Rome, Rome, Italy Tropical Medicine Graduate Program, Academic Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand Aberystwyth University, Institute of Biological, Environmental & Rural Sciences (IBERS), Wales, Aberystwyth Ceredigion, United Kingdom McGill University, Institute of Parasitology, Ste-Anne-de-Bellevue, QC, Canada University of Glasgow, Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, Glasgow, United Kingdom Wellcome Sanger Institute, Parasites and Microbes, Cambridge, United Kingdom University of Zurich, Institute of Parasitology, Vetsuisse Faculty, Zurich, Switzerland Universidad de la República, Facultad de Medicina, Departamento de Genetica, Montevideo, Uruguay Queen's University Belfast, School of Biological Sciences, Northern Ireland, Belfast, United Kingdom Leiden University Medical Center, Parasitology, Leiden, Netherlands Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, Serbia Utrecht University, Faculty of Veterinary Medicine, Biomolecular Health Sciences, Utrecht, Netherlands Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand James Cook University, Australian Institute of Tropical Health and Medicine, Cairns, QLD, Australia University of Glasgow, Wellcome Centre for Integrative Parasitology, Institute of Infection, Immunity and Inflammation, Glasgow, United Kingdom Universitat de València, Departamento Farmacia y Tecnología Farmacéutica y Parasitología, Área de Parasitología, Valencia, Burjsassot, Spain Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic Czech Republic, Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Prague, Czech Republic University of Pennsylvania School of Veterinary Medicine, Pathobiology, Philadelphia, PA, United States Laboratory of Helminthology, Faculty of Microbiology, University of Costa Rica, San Pedro Montes de Oca, Costa Rica Medical University of Vienna, Institute of Specific Prophylaxis and Tropical Medicine, Vienna, Austria Department of Pre-clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand Swiss Tropical and Public Health Institute, Allschwil, Switzerland University of Basel, Basel, Switzerland Export Date: 14 February 2023 Correspondence Address: Buck, A.H.; The University of Edinburgh, United Kingdom; email: Correspondence Address: Nejsum, P.; Department of Clinical Medicine, Denmark; email: Correspondence Address: Hoffmann, K.F.; Aberystwyth University, Ceredigion, United Kingdom; email:
Uncontrolled Keywords: electron microscopy, EV guidelines, EV reporting, extracellular vesicles, helminths, parasites
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
Q Science / természettudomány > QR Microbiology / mikrobiológia > QR180 Immunology / immunológia
R Medicine / orvostudomány > RS Pharmacy and materia medica / gyógyszerészet, gyógyászati eszközök
Depositing User: MTMT SWORD
Date Deposited: 04 Apr 2024 09:50
Last Modified: 04 Apr 2024 09:50

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