Kadir, E. R. Doganhan and Dundar, Devrim and Uzuner, Huseyin (2023) Distribution of virulence determinants among Escherichia coli ST131 and its H30/H30-Rx subclones in Turkey. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 70 (1). pp. 47-51. ISSN 1217-8950
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Abstract
Extraintestinal pathogenic Escherichia coli (ExPEC) is the leading pathogen in urinary tract infection. In recent years multidrug-resistant B2-ST131 E. coli clonal group has disseminated worldwide. The ST131 and its subclones H30 and H30-Rx have been identified only in a few studies from Turkey. The aim of this study is to investigate the presence of ST131 and its subclones and to analyze their adhesin virulence genes and antimicrobial resistance. A total of 250 urinary ExPEC isolates were included in the study. Resistance rates of 16 antimicrobial agents were determined by disk-diffusion. Multidrug-resistance and ESBL production were analyzed. Altogether 8 adhesin genes were investigated namely, papAH, fimH, sfa/focDE, focG, afa/draBC, iha, bmaE and gafD. A total of 39 ST131 isolate were determined and 33 (84.6%) were multidrug-resistant. ESBL production was detected in 34 (87.2%) ST131 and 61 (28.9%) of non-ST131 strains. In our study, we found a strong correlation between ST131 strains and fimH, iha, afa/draBC, papAH virulence determinants. Twenty-nine (85.3%) of 34 ST131-O25b-H30 isolates were identified as H30-Rx. All the papAH gene positive isolates were identified within ST131-O25b-H30-Rx lineage. Non-H30-Rx isolates within H30 isolates were identified as pattern 2. Almost 16% of the isolates were identified as ST131 regardless of clinical syndrome and approximately 34% of the multidrug-resistant isolates were H30-Rx subclone. We report H30-Rx as the dominant subclone of ST131 in our study. Imipenem, fosfomycin and nitrofurantoin proved to be the most effective agents according to antibiotic resistance patterns of both ST131 and non-ST131 E. coli strains.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Scientific and Technological Research Council of Turkey (TUBITAK); [215S136] Funding text: This study was presented at 7th Congress of European Microbiologists (FEMS 2017) in Valencia, Spain as poster presentation at 10/07/2017. This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) [grant number 215S136] . We are grateful to Prof. Dr. James R. Johnson and Dr. Brian Johnston for their technical advices and positive control DNAs of the E. coli strains 2H16, V27 and PM9 and positive control strains of the E. coli MVAST0004, MVAST0016 and MVAST0020. |
| Uncontrolled Keywords: | RESISTANCE; immunology; SPREAD; Escherichia coli; emergence; Molecular Epidemiology; URINARY-TRACT-INFECTIONS; BETA-LACTAMASE; ST131; H30; SEQUENCE TYPE ST131; H30-Rx; CLONAL GROUPS; |
| Subjects: | Q Science / természettudomány > QR Microbiology / mikrobiológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 15 Apr 2024 05:36 |
| Last Modified: | 15 Apr 2024 05:36 |
| URI: | https://real.mtak.hu/id/eprint/192513 |
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