Yousefi, Shaghayegh and Nazari, Maryam and Ramazanzadeh, Rashid and Sahebkar, Amirhossein and Safarzadeh, Elham and Khademi, Farzad (2023) Association of carbapenem and multidrug resistance with the expression of efflux pump-encoding genes in Pseudomonas aeruginosa clinical isolates. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 70 (2). pp. 161-166. ISSN 1217-8950
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Abstract
Efflux pumps play an important role in the emergence of antibiotic-resistant Pseudomonas aeruginosa strains. The present study aimed to assess the expression of the MexAB-OprM, MexCD-OprJ, MexEFOprN, and MexXY-OprM efflux pumps in carbapenem-resistant and multidrug-resistant (MDR) P. aeruginosa strains isolated from clinical specimens between June 2019 and January 2022 in Ardabil city. The presence of efflux pump-encoding genes, i.e. mexA, mexC, mexE, and mexY, was assessed using the polymerase chain reaction (PCR) technique in 48 carbapenem-resistant and MDR P. aeruginosa strains. Real-time reverse transcription PCR was employed to evaluate the expression levels of mexA, mexC, mexE, and mexY genes. All 48 carbapenem-resistant and MDR P. aeruginosa strains harbored efflux pump-encoding genes including mexA, mexC, mexE, and mexY according to the PCR results. Overexpression of the MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY-OprM efflux pumps was detected in 75% (n = 36), 83.3% (n = 40), 10.4% (n = 5) and 41.6% (n = 20) of the clinical isolates of P. aeruginosa, respectively. This study revealed that the presence and overexpression of efflux pumps are associated with the emergence of carbapenem-resistant and MDR P. aeruginosa strains. Therefore, research on efflux pump inhibitors of P. aeruginosa will be a worthwhile endeavor to increase the clinical efficiency of available antibiotics and prevent ensuing treatment failure.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Ardabil University of Medical Sciences, Iran Funding text: This research has been financially supported by the Vice Chancellor for Research, Ardabil University of Medical Sciences, Iran, and approved by Regional Research Ethics Committee (approval ID: IR.ARUMS.REC.1400.322). |
| Uncontrolled Keywords: | MUTATIONS; immunology; Overexpression; Pseudomonas aeruginosa; carbapenem; efflux pump; ANTIBIOTIC-RESISTANCE; MEXEF-OPRN; |
| Subjects: | Q Science / természettudomány > QR Microbiology / mikrobiológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 16 Apr 2024 07:33 |
| Last Modified: | 30 Jun 2024 23:15 |
| URI: | https://real.mtak.hu/id/eprint/192602 |
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