High-risk clonal lineages among extended-spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae from urban and rural stagnant water samples in Tunisia

Romdhani, Amel and Cheriet, Sarah and Abbassi, Mohamed Salah and Lengliz, Sana and Hynds, Paul and Boutiba-ben, Boubaker Ilhem and Landolsi, Ramzi Boubaker (2023) High-risk clonal lineages among extended-spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae from urban and rural stagnant water samples in Tunisia. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 70 (4). pp. 304-310. ISSN 1217-8950

Preview

Text 030-article-p340.pdf Download (892kB) | Preview

Abstract

This study sought to investigate the occurrence and subsequently to characterize extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae from urban and rural stagnant water samples during the wet season (December to February) in several regions of northern Tunisia. From 56 stagnant water samples, 14 ESBL-producing Enterobacteriaceae were recovered, including 9 Escherichia coli, 3 Klebsiella pneumoniae, and 2 K. oxytoca. Most isolates were multidrug-resistant, with ESBL production primarily encoded by bla(CTX-M-15) (n = 8) and bla(CTX-M-1) (n = 4) followed by bla(CTX-M-55) (n = 1) and bla(TEM-26) (n = 1). One K. pneumoniae isolate co-harbored bla(KPC) and bla(CTX-M-15) genes. Class 1 integrons were detected in 4 isolates, however, sul1, sul2, and aac(60)-Ib-cr genes were detected in eleven, two, and four isolates, respectively. The nine E. coli isolates belonged to seven sequence types namely, B2/ST131 (3 isolates), A/ST164, A/ST10, A/ST224, A/ST38, A/ST155, and A/ST69 (each of them one isolate). The three K. pneumoniae isolates were assigned to three sequence types: ST101, ST405 (harboring CTX-M-15 and KPC), and ST1564. Overall, the phenotypic and genotypic traits of collected isolates mirror the molecular epidemiology of ESBL-producing enterobacteria in Tunisia and highlight the potential role of stagnant water in both urban and rural areas as a reservoir of ESBL-producing Enterobacteriaceae.

Actions (login required)

Edit Item