Altatás napos korban víziszárnyas fajokban – kihívások és megoldások = Anaesthesia of Waterfowl Species at Day-Old Age – Challenges and Solutions

Buda, Kitti and Rohn, Emese and Pazár, Péter and Végi, Barbara and Lehoczky, István and Liptói, Krisztina (2024) Altatás napos korban víziszárnyas fajokban – kihívások és megoldások = Anaesthesia of Waterfowl Species at Day-Old Age – Challenges and Solutions. MAGYAR ÁLLATORVOSOK LAPJA, 146 (5). pp. 285-290. ISSN 0025-004X

Text 285_290_Buda_baromfi.pdf Restricted to Repository staff only Download (1MB) | Request a copy

Abstract

Madarakban a nőivarú egyedek genetikai anyagának megőrzésének egyik módszere a naposkori petefészekszövet-átültetés. Jelen közleményben a szerzők célja a transzplantációhoz szükséges altatás kihívásainak és a megfelelő protokoll kidolgozásának bemutatása naposlibában és naposkacsában. A naposcsibéknél alkalmazott módszerből kiindulva lúdban a megemelt dózisú ketamin és xylazin midazolammal kiegészítve eredményezett megfelelő mélységű anesztéziát akkor, ha 70%-át izomba, majd 10 perc elteltével a maradék 30%-ot vénásan adagolták. Ugyanez a kombináció kacsában kizárólag iv. volt hatékony. Ezeket a protokollokat alkalmazva elvégezhető az operáció. Background: The most widely used method for avian in vitro gene conservation is the cryopreservation of semen. This technique is only suitable for the preservation of male genome; therefore, an alternative method is needed for the conservation of female genetic material. For this purpose, cryopreservation and transplantation of gonadal tissue at day-old age can be suitable. In domestic fowl donor-derived progeny has been regained from the transplantation of frozen /thawed ovary. In duck donor-derived offspring was obtained from transplantation of native ovarian tissue, however, in geese no similar results have been published. Objectives: For performing the ovarian transplantation, a short, 15 minutes long anaesthesia is needed. In the literature there is very few information about the anaesthesia of waterfowl species at day-old age. The aim of this study is to present the challenges of the anaesthesia of day-old geese and duck. Materials and Methods: For the anaesthesia of day-old Grey Landes and White Hungarian geese and mulard ducks the authors first applied the protocol developed for day-old chicks (combination of ketamine 10 mg/kg and xylazine 2 mg/ kg, administered intramuscularly (im.)) but this only resulted mild sedation. Then the dosage of the medications was raised to 15 mg/kg of ketamine and 3 mg/kg of xylazine. For the maintenance no anaesthetic machine was used, only a piece of cotton infused with isoflurane, administered drop by drop. Subsequently, the combination of ketamine (15 mg/kg), xylazine (3 mg/kg) and midazolam (2 mg/kg) was administered im., then after 10 minutes the dose was repeated, which led to cardiac arrest probably due the cumulation of drugs in the adipose tissue. After that both in geese and ducks part the combination was given im., then after 10 minutes the leftover intravenously (iv.). Results and Discussion: Administration of the 70% of the combination im. then after 10 minutes the 30% iv. resulted the required level of anaesthesia. In case of ducks, the authors found that applying the dose only intravenously was the most suitable technique, because then the animals only need to be injected once. Developing the anaesthesia protocol can make it possible to perform the ovarian transplantation in day-old waterfowl species.

Actions (login required)

Edit Item