Voltage clamp measurement set-up for investigation of membrane parameters

Véró, Mihály (1974) Voltage clamp measurement set-up for investigation of membrane parameters. A Magyar Tudományos Akadémia Tihanyi Biológiai Kutatóintézetének évkönyve, 41. pp. 111-117. ISSN 0365-3005

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Abstract

According to the voltage clamp method, measurement is performed by the aid of the two intracellular glass microelectrodes applied to the nerve cell. One of the electrodes is used to record the changing voltage of the cell membrane. This voltage and a defined command step voltage are applied to a summing network, and the current corresponding to the sum is applied to the other (current) electrode. Membrane voltage is thus constant with good approximation, and the ion currents flowing during the measurement may be recorded by a suitable current-voltage converter. In the paper, the basic measurement principle, the design criteria of the amplifier parameters, the d ata of the double step square wave generator and the detailed circuit description used for actual measurements are presented.

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