Fluorescence microscopy and micro- spectrofluorimetry of the monoamines in the brain of Locusta migratoria migratorioides R. F. (Insecta, Orthoptera) with special regard to the protocerebrum

B.-Muskó, Ilona and Zs.-Nagy, Imre and Deák, György (1973) Fluorescence microscopy and micro- spectrofluorimetry of the monoamines in the brain of Locusta migratoria migratorioides R. F. (Insecta, Orthoptera) with special regard to the protocerebrum. A Magyar Tudományos Akadémia Tihanyi Biológiai Kutatóintézetének évkönyve, 40. pp. 85-94. ISSN 0365-3005

Preview

Text 86_cut_MTA_TihanyiBiologiaiKutIntEvkonyve_40.pdf Download (4MB) | Preview

Abstract

The cerebral ganglion of the Locusta migratoria migratorioides was in vestigated except the optic lobe. Only green fuorescence was found after Falck-Hillarp formaldehyde reaction in th e regions investigated. In the mushroom body green fluorescence was observed in the pedunculus, alpha and beta lobes, but was not in the globuli cell layer and the calyx neuropile. The most intense fluorescence was detected in the central body. Varicose, fluorescing fibres are present now and then in the protocerebral neuropile as well as in the tritocerebrum, in some places they are grouped. We failed to observe any fluorescing structures in the deutocerebrum. The microspectrofluorimetric analysis of the fluorophore revealed that the fluorescence originates only in catecholamines, fluorophores of catecholamines and serotonin do not occur simultaneously in the regions investigated. The exposure to HCl-vapours proved that the catecholamines consist mainly of dopamine, in accordance with other species.

Actions (login required)

Edit Item