Incorporation of 1-¹⁴C stearic acid and 1-¹⁴C linolenic acid into the liver lipids of the carp (Cyprinus carpio L.)

Herodek, Sándor (1973) Incorporation of 1-¹⁴C stearic acid and 1-¹⁴C linolenic acid into the liver lipids of the carp (Cyprinus carpio L.). A Magyar Tudományos Akadémia Tihanyi Biológiai Kutatóintézetének évkönyve, 40. pp. 191-195. ISSN 0365-3005

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Abstract

Liver slices of carps were incubated at 28°C and 8°C with 1-14C stearic and 1-¹⁴C linolenic acids for 30 min. From stearic acid at 28°C 82.9 ± 13.2, at 8°C 51.2 ± 6.4, from linolenic acid at 28°C 113.0 ± 8.6 at 8°C 86.3 ± 9.3 nannomole fatty acid was esterified by one gram liver. Temperature had no detectable effect on the distribution of fatty acids in the lipid classes. Radioactivity of diglycerides in liver slices first incubated with labeled stearic acid decreased to its half only after a two-hour reincubation in inactive medium. This indicates some other role of diglycerides in addition to the p articipation in the rap id process of triglyceride synthesis. The amount of free fatty acids bound by liver slices was independent of the type of fatty acid given and of the temperature, but decreased to a very low level during reincubation in inactive medium.

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