Comparative nutrient agar studies on the quantitative survey of saprophytic water microorganisms

Oláh, János and Vásárhelyi, Réka (1970) Comparative nutrient agar studies on the quantitative survey of saprophytic water microorganisms. A Magyar Tudományos Akadémia Tihanyi Biológiai Kutatóintézetének évkönyve, 37. pp. 235-246. ISSN 0365-3005

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Abstract

1. Significant differences have been observed between culture media used by us for counting saprophytic microorganisms. On the basis of results obtained by comparative analysis, for the waters in Hungary the sodium-caseinate agar proved to be the most suitable for counting saprophytic microorganisms. This medium yielded the highest number for bacteria, the colonies were small, did not liquefy and the chromogenic bacteria occur in them in greater numbers. 2. It was interesting to note that on nutrient media containing only natural substrates and salts a large number of bacteria were able to develop. The slow rate of growth of the colonies and the difficulty in their counting make them suitable, primarily, for “special” investigations. 3. The authors in studying the microflora of waters by direct and indirect methods found that significant differences exist between the results of the two methods, which, according to them, are mainly due to the selecting property of the employed nutrient media as against the supposed high percentage of dead organisms counted on the membrane filter.

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