Comparative study of recognized (classic) and modified impregnation methods on the ganglions of Anodonta cygnea L.

Gubicza, András and Zs.-Nagy, Imre (1964) Comparative study of recognized (classic) and modified impregnation methods on the ganglions of Anodonta cygnea L. A Magyar Tudományos Akadémia Tihanyi Biológiai Kutatóintézetének évkönyve, 31. pp. 15-21. ISSN 0365-3005

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Abstract

Authors have silvered the ganglions of Anodonta cygnea L. with several generally known impregnation methods. The more rapid freezing procedures, the silvering processes of paraffin sections and the total impregnation methods suited for series sectioning were tested. From the silvering methods of the frozen sections the shortened J abonero the Jabonero-Bielschowsky, Cajax, Bielschowsky, Bielschowsky- Ábrahám and Cauna methods, from the impregnation rae'thodies of paraffin sections the Cajal I., Cajal IV., Weber, Golgi quick, Cajal—Faworsky methods were tried out. The above methods were repeatedly tested according to specifications but none of them gave satisfactory results. Therefore several impregnation methods were modified by the authors. In the individual methods the AgNO₃ concentration, the incubation period and the reduction process was changed. In the Rowell method the pH of the demethylpyridine silver nitrate was modified while with the Cajal I method FeSO₄ preliminary treatment was applied. The impregnation of the frozen sections with the modification of Cajal’s process is moderately suited (Fig. 1) to make the fibres visible while the modified Cattna method is excellently suited (Fig. 2). The R owell method in its original form silvers the nerve cells of the mussel ganglions not at all, while the fibres very poorly (Fig. 3); but the nerve fibres of ganglions silvered according to the authors modification resulted in beautiful preparations (Fig .4). From the block impregnation methods Cajal IV in its modified form is suited for the fibres to be made visible (Fig. 5) while Cajal I. after FeSO₄ pretreatment proved to be excellent both for the impregnation of ganglion cells and appendices.

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