Binding Constant of V(IV)O to Transferrin

Kiss, Tamás and Jakusch, Tamás and Bouhsina, S. and Sakurai, H. and Enyedy, Éva Anna (2006) Binding Constant of V(IV)O to Transferrin. EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, 2006 (18). pp. 3607-3613. ISSN 1434-1948

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Abstract

The interactions of bis-VIVO-1,2-dimethyl-3-hydroxy-4(1H)-pyridinone (DHP) with apotransferrin (apoTF) were followed by means of electron paramagnetic resonance spectroscopy (EPR) at room temperature and at liquid nitrogen temperature. Partial ligand displacement between DHP and apoTF was observed, and the reaction was used to determine the binding constant of VIVO to apoTF. A value of log K = 14.3 ± 0.6 was obtained for the binding of the first VIVO to apoTF. In order to confirm the validity of the binding constant, UV spectroscopy was used to monitor the displacement reaction between VIVO–apoTF and nitrilotriacetic acid (NTA). The difference between the binding constants obtained by the two different methods (ca. one log unit) was explained in terms of the possible ternary complex formation between VIVO–apoTF and the displacing ligand. The binding constants obtained exhibit a reasonably good agreement with the linear free energy relationship (LFER) estimation.

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