Kinase-heparin antagonism in vitro

Csefkó, István and Gerendás, Mihály and Udvardy, Miklós (1948) Kinase-heparin antagonism in vitro. A Magyar Tudományos Akadémia Tihanyi Biológiai Kutatóintézetének évkönyve, 18. pp. 186-192. ISSN 0365-3005

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Abstract

By heparin toluidine-blue colour-reaction we established that heparin, or a heparin-like substance dissolves from kinase in Ringer solution. In a similar way we also showed that the kinase thus freed of heparin could bind heparin from a more concentrated heparin solution. We found that the kinase decreased equally the normal thrombin activating capacity of the blood, and that increased by heparin. It is suggested that the role of kinase in the clotting of blood is an inhibition of thrombin inactivation, whereby the thrombin level is raised to the level necessary to bring about clotting. We established an analogy between the histamine-heparin antagonism found in our earlier experiments and the present investigation. Our observations stand close to the conceptions of Fuchs, Del Baere, and Howell (l. c.) principally, as opposed to those usually found in the literature, namely, that the function of kinase consists in the activation of prothrombin.

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