Mihók, Edit and Polgári, Dávid and Lenykó-Thegze, Andrea and Makai, Diána and Fábián, Attila and Ali, Mohammad and Kis, András and Sepsi, Adél and Sági, László (2024) Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids. FRONTIERS IN PLANT SCIENCE, 15. ISSN 1664-462X
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Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids_2024.pdf - Published Version Available under License Creative Commons Attribution. Download (18MB) | Preview |
Abstract
Incorporating the centromere-specific histone H3 protein CENH3 into the centromeric nucleosomes is indispensable for accurate centromere function and balanced chromosome segregation in most eukaryotes, including higher plants. In the cell nuclei of interspecific hybrids, divergent centromeric DNAs cohabit and lead the corresponding parental chromosomes through the mitotic and meiotic cell divisions. Depending on the transmission of the parental chromosomes carrying the CENH3-encoding genes, CENH3 proteins from one or both parents may be present in these hybrids. The incorporation of parental CENH3 proteins into the divergent centromeres and their role in the chromosome elimination process in interspecific hybrids is still poorly understood. Here, we produced wheat × barley F1 hybrids that carried different combinations of barley chromosomes with genes encoding for either one (αCENH3) or both barley CENH3 protein variants (α– and βCENH3). We generated specific antibodies distinguishing between the wheat CENH3 proteins and barley αCENH3 and applied them together with FISH probes to detect the precise pattern of parental CENH3 deposition into the wheat and barley centromeric nucleosomes. Analysis of somatic and meiotic nuclei of the wheat × barley hybrids revealed the plasticity of the maternal (wheat) CENH3 proteins to become incorporated into the paternal (barley) centromeric nucleosomes. However, no evidence for paternal CENH3 plasticity was detected in this study. The significance of the unilateral centromere plasticity and possible patterns of CENH3 incorporation into centromeres in interspecific hybrids are discussed.
Item Type: | Article |
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Uncontrolled Keywords: | centromeric DNA, chromosome elimination, Hordeum vulgare, interspecific hybridization, Triticum aestivum |
Subjects: | Q Science / természettudomány > Q1 Science (General) / természettudomány általában S Agriculture / mezőgazdaság > S1 Agriculture (General) / mezőgazdaság általában |
Depositing User: | Dr. András Kis |
Date Deposited: | 18 Sep 2024 13:13 |
Last Modified: | 18 Sep 2024 13:13 |
URI: | https://real.mtak.hu/id/eprint/205162 |
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