Maciejewska-Prończuk, Julia and Oćwieja, Magdalena and Żeliszewska, Paulina and Wasilewska, Monika and Ungor, Ditta and Csapó, Edit and Szyk-Warszyńska, Lilianna and Gajewska, Marta and Charzanowska, Agnieszka and Dobrzyńska, Joanna and Ivashchenko, Inna and Matras-Postołek, Katarzyna and Adamczyk, Zbigniew (2025) Fluorescent gold nanoclusters stabilized by lysozyme: Synthesis and deposition kinetics on silica substrates. JOURNAL OF LUMINESCENCE, 277. No.-120912. ISSN 0022-2313
|
Text
1-s2.0-S0022231324004769-main.pdf - Published Version Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
Gold nanoclusters suspension were effectively synthesized under alkaline conditions in a chemical reduction process involving gold(III) chloride trihydrate and lysozyme (LYZ) molecules. Their size determined by high-resolution transmission electron microscopy (HR-TEM) was equal to 1.9 ± 0.5 nm. The nanoclusters, referred to as LYZ-Au NCs, were stable at pH below 4 and above 8, exhibiting a hydrodynamic diameter between 8 and 11 nm. The isoelectric point of LYZ-Au NCs appeared at pH 5.0. The suspension showed a pronounced fluorescence characterized by the red-emitting band at 668 nm. The deposition kinetics and stability of LYZ-Au NCs on bare and poly(diallyldimethylammonium chloride) (PDADMAC)-modified silica sensors were studied using quartz crystal microbalance (QCM). The influence of ionic strength, pH, and suspension concentration on the kinetics of LYZ-Au NCs deposition was determined. The significant increase in the maximum coverage of LYZ-Au NCs with ionic strength was attributed to the decreasing range of electrostatic interactions between deposited clusters. Atomic force microscopy (AFM) confirmed the formation of homogeneous layers of LYZ-Au NCs with controlled coverage on bare silica at pH 3.5 and PDADMAC-modified silica. It was shown by confocal microscopy investigations, that these layers also exhibited pronounced fluorescent properties.
Item Type: | Article |
---|---|
Subjects: | Q Science / természettudomány > QD Chemistry / kémia |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 29 Sep 2024 17:33 |
Last Modified: | 29 Sep 2024 17:33 |
URI: | https://real.mtak.hu/id/eprint/206388 |
Actions (login required)
![]() |
Edit Item |