The effect of paramagnetic center of 2.03 average g-value on D-glyceraldehyde-3-phosphate dehydrogenase

Elek, G and Keleti, Tamás (1971) The effect of paramagnetic center of 2.03 average g-value on D-glyceraldehyde-3-phosphate dehydrogenase. ACTA BIOCHIMICA ET BIOPHYSICA HUNGARICA, 6 (2). pp. 107-121. ISSN 0237-6261

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Abstract

Pairwise coupled reactions of fructose-1,6-bisphosphate aldolase and sn-glycerol-3-phosphate dehydrogenase, 3-phosphoglycerate kinase and D-glyceraldehyde-3-phosphate dehydrogenase,triosephosphate isomerase and sn-glycerol- 3-phosphate dehydrogenase have been detected by micro spectrophotometry in single crystals obtained from myogen A in the presence of polyethylene glycol. Microspectro- photometric measurements with polarized light demonstrate that the protein molecules are oriented and that NADH is bound with a definite orientation to the dehydrogenases within the crystal.

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