Keleti, Tamás and Berni, R and Kazinczyné Vas, Mária and Mozzarelli, A and Rossi, GL (1989) Coupled enzymatic reactions measured in a single protein crystal from myogen A. ACTA BIOCHIMICA ET BIOPHYSICA HUNGARICA, 24 (1-2). pp. 15-23. ISSN 0237-6261
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Abstract
Pairwise coupled reactions of fructose-1,6-bisphosphate aldolase and sn-glycerol-3-phosphate dehydrogenase, 3-phosphoglycerate kinase and D-glyceraldehyde-3-phosphate dehydrogenase,triosephosphate isomerase and sn-glycerol- 3-phosphate dehydrogenase have been detected by micro spectrophotometry in single crystals obtained from myogen A in the presence of polyethylene glycol. Microspectro- photometric measurements with polarized light demonstrate that the protein molecules are oriented and that NADH is bound with a definite orientation to the dehydrogenases within the crystal.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RABBITS; ENZYMES; MUSCLE; animal; nonhuman; animal cell; CRYSTALLIZATION; In Vitro; Spectrophotometry; Rabbit; triosephosphate isomerase; phosphoglycerate kinase; Glycolysis; NAD; glyceraldehyde 3 phosphate dehydrogenase; fructose bisphosphate aldolase; Muscle Proteins; glycerol 3 phosphate dehydrogenase; protein crystallization; myogen a; |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 28 Oct 2024 09:50 |
| Last Modified: | 28 Oct 2024 09:50 |
| URI: | https://real.mtak.hu/id/eprint/208060 |
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