Association of insulin-like growth factor-1 gene with maternal growth, reproduction and conformation traits in Harnali sheep

Kumar, Sandeep and Dahiya, Satpal and Magotra, Ankit and Bangar, Y.C. (2024) Association of insulin-like growth factor-1 gene with maternal growth, reproduction and conformation traits in Harnali sheep. ACTA VETERINARIA HUNGARICA, 72 (3). pp. 186-194. ISSN 0236-6290

Text 004-article-p186.pdf - Published Version Restricted to Repository staff only Download (839kB) | Request a copy

Abstract

The present study explored the association between a point mutation (g.857G>A) in the IGF-1 gene and performance traits in Harnali Sheep. Genomic DNA was extracted from 110 Harnali sheep using an Automated Maxell RSC DNA/RNA purification system. A 294 bp fragment, covering the 50 flanking region of the IGF-1 gene, was amplified with specific primers. The resulting PCR product, harboring the g.857G>A mutation, was digested with the Hae II enzyme to determine genotypes. The frequency of the A allele of the IGF-1 gene was higher (0.59) in the studied Harnali sheep population, with the GA genotype being the most prevalent (0.64). The presence of the A allele was associated with higher body weights, suggesting its potential as a selection criterion for improving flock performance. Furthermore, the GA genotype was linked to a lower average age at first lambing (727.93 days) compared to the GG genotype (753.08 days). The A allele was linked to higher body weights, while the GA genotype was associated with a lower age at first lambing. These findings highlight the potential of these genetic markers for improving breeding strategies and enhancing flock performance. However, validation on larger samples is warranted.

Actions (login required)

Edit Item