TFIIS is required for reproductive development and thermal adaptation in barley

IMTIAZ, AHMAD and Kis, András and Verma, Radhika and Szádeczky-Kardoss, István and Szaker, Henrik Mihály and Pettkó-Szandtner, Aladár and Silhavy, Dániel and Havelda, Zoltán and Csorba, Tibor (2024) TFIIS is required for reproductive development and thermal adaptation in barley. PLANT CELL REPORTS, 43 (11). No.-260. ISSN 0721-7714

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Abstract

Regulation of transcriptional machinery and its adaptive role under different stress conditions are studied extensively in the dicot model plant Arabidopsis, but our knowledge on monocot species remains elusive. TFIIS is an RNA polymerase II-associated transcription elongation cofactor. Previously, it was shown that TFIIS ensures efficient transcription elongation that is necessary for heat stress survival in A. thaliana. However, the function of TFIIS has not been analysed in monocots. In the present work, we have generated and studied independent tfIIs-crispr-mutant barley lines. We show that TFIIS is needed for reproductive development and heat stress survival in barley. The molecular basis of HS-sensitivity of tfIIs mutants is the retarded expression of heat stress protein transcripts, which leads to late accumulation of HSP chaperones, enhanced proteotoxicity and ultimately to lethality. We also show that TFIIS is transcriptionally regulated in response to heat, supporting a conserved adaptive function of these control elements for plant thermal adaptation. In sum, our results are a step forward for the better understanding of transcriptional machinery regulation in monocot crops.

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