Akhtar, Parveen and Jana, Sanjib and Lambrev, Petar and Tan, Howe-Siang (2024) Inhomogeneous energy transfer dynamics from iron-stress-induced protein A to photosystem I. FRONTIERS IN PLANT SCIENCE, 15. No.-1393886. ISSN 1664-462X
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Abstract
Cyanobacteria respond to iron limitation by producing the pigment-protein complex IsiA, forming rings associated with photosystem I (PSI). Initially considered a chlorophyll-storage protein, IsiA is known to act as an auxiliary light-harvesting antenna of PSI, increasing its absorption cross-section and reducing the need for iron-rich PSI core complexes. Spectroscopic studies have demonstrated efficient energy transfer from IsiA to PSI. Here we investigate the room-temperature excitation dynamics in isolated PSI–IsiA, PSI, IsiA monomer complexes and IsiA aggregates using two-dimensional electronic spectroscopy. Cross analyses of the data from these three samples allow us to resolve components of energy transfer between IsiA and PSI with lifetimes of 2— 3 ps and around 20 ps. Structure-based Förster theory calculations predict a single major timescale of IsiA-PSI equilibration, that depends on multiple energy transfer routes between different IsiA subunits in the ring. Despite the experimentally observed lifetime heterogeneity, which is attributed to structural heterogeneity of the supercomplexes, IsiA is found to be a unique, highly efficient, membrane antenna complex in cyanobacteria.
Item Type: | Article |
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Additional Information: | Funding Agency and Grant Number: National Research, Development and Innovation Fund [NKFI FK-139067, ANN-144012, 2018-1.2.1-NKP-2018-00009]; Hungarian Research Network [SA-76/2021]; Singapore Ministry of Education Academic Research Fund [RG9/21, MOE-T2EP50122-0022]; Scientia Amabilis Foundation Funding text: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The work was supported by grants from the National Research, Development and Innovation Fund (NKFI FK-139067 to PA and ANN-144012 and 2018-1.2.1-NKP-2018-00009 to PL), the Hungarian Research Network (SA-76/2021 to PA) and Singapore Ministry of Education Academic Research Fund (RG9/21 and MOE-T2EP50122-0022) to HT. PA acknowledges support from the Scientia Amabilis Foundation. |
Uncontrolled Keywords: | cyanobacteria, excitation energy transfer, ultrafast spectroscopy, iron-stress, photophysics, photosystem I, light-harvesting, iron stress |
Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia Q Science / természettudomány > QK Botany / növénytan Q Science / természettudomány > QR Microbiology / mikrobiológia |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 23 Jan 2025 14:11 |
Last Modified: | 23 Jan 2025 14:11 |
URI: | https://real.mtak.hu/id/eprint/214237 |
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