Alzyoud, Elham and Németh, Dóra and Vedelek, Viktor and Szögi, Titanilla and Tóth, Viktória Petra and Krecsmarik, Mónika and Ábrahám, Edit and Lipinszki, Zoltán and Sinka, Rita (2024) Versatile gamma-tubulin complexes contribute to the dynamic organization of MTOCs during Drosophila spermatogenesis. COMMUNICATIONS BIOLOGY, 7 (1). No.-1385. ISSN 2399-3642
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Abstract
The initiation of microtubule formation is facilitated by gamma-tubulin and gamma-Tubulin Ring Complex (gamma-TuRC) in various microtubule-organizing centers (MTOCs). While the heterogeneity of tissue-specific MTOCs and gamma-TuRC in Drosophila testis has been described, their molecular composition and physiological significance are poorly understood. We investigated the testis-specific distribution and biochemical interaction of the canonical gamma-TuRC proteins Grip163 and Grip84. We found that while Grip163 is present on the centrosome and basal body, Grip84 localizes to the centrosome and Golgi in spermatocytes and colocalizes with the testis-specific gamma-Tubulin complexes (t-gamma-TuC) at the basal body, apical nuclear tip, and near the elongated mitochondria after meiosis. We also showed the apical nuclear tip localization of some gamma-TuRC interacting partners and proved their binding to t-gamma-TuC proteins. These results highlight and prove the importance of the different gamma-TuRCs in organizing the diverse MTOCs present during the extensive rearrangement of cell organelles during the spermatogenesis of Drosophila. The heterogeneous distribution of various gamma-tubulin binding complexes contributes to the formation of diverse microtubule-organizing centers and the comprehensive rearrangement of cellular organelles during spermatogenesis in Drosophila.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Hungarian Science Foundation [2P40OD010949]; NIH [K132155]; Hungarian Science Foundation [2022-2.1.1-NL-2022-00008]; National Laboratory for Biotechnology Funding text: We are grateful to all members of the Sinka lab. We are indebted to David M. Glover, Helen White-Cooper, Sean Munro and Jordan Raff for fly stocks and reagents; for the Drosophila Genomics Resource Center (supported by NIH grant 2P40OD010949) for cDNA clones and to Ferenc Jankovics for critical reading of the manuscript. This work was supported by Hungarian Science Foundation (K132155) to R.S. and the National Laboratory for Biotechnology (2022-2.1.1-NL-2022-00008) to Z.L. |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia Q Science / természettudomány > QH Natural history / természetrajz > QH426 Genetics / genetika, örökléstan |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 23 Jan 2025 14:26 |
| Last Modified: | 23 Jan 2025 14:26 |
| URI: | https://real.mtak.hu/id/eprint/214239 |
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