The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair development

Benyó, Dániel and Bato, Emese and Faragó, Dóra and Rigó, Gábor and Racskóné Domonkos, Ildikó and Labhane, Nitin and Zsigmond, Laura and Prasad, Melvin and Nagy, István and Szabados, László (2024) The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair development. FRONTIERS IN PLANT SCIENCE, 14. No.-1221519. ISSN 1664-462X

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Abstract

Introduction Zinc finger protein 3 (ZFP3) and closely related C2H2 zinc finger proteins have been identified as regulators of abscisic acid signals and photomorphogenic responses during germination. Whether ZFP3 and related ZFP factors regulate plant development is, however, not known.Results ZFP3 overexpression reduced plant growth, limited cell expansion in leaves, and compromised root hair development. The T-DNA insertion zfp3 mutant and transgenic lines with silenced ZFP1, ZFP3, ZFP4, and ZFP7 genes were similar to wild-type plants or had only minor differences in plant growth and morphology, probably due to functional redundancy. RNAseq transcript profiling identified ZFP3-controlled gene sets, including targets of ABA signaling with reduced transcript abundance. The largest gene set that was downregulated by ZFP3 encoded regulatory and structural proteins in cell wall biogenesis, cell differentiation, and root hair formation. Chromatin immunoprecipitation confirmed ZFP3 binding to several target promoters.Discussion Our results suggest that ZFP3 and related ZnF proteins can modulate cellular differentiation and plant vegetative development by regulating the expression of genes implicated in cell wall biogenesis.

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