Nath Bhaumik, Kaushik and Spohn, Réka and Dunai, Anett and Daruka, Lejla and Olajos, Gábor and Zákány, Florina and Hetényi, Anasztázia and Pál, Csaba and Martinek, Tamás (2024) Chemically diverse antimicrobial peptides induce hyperpolarization of the E. coli membrane. COMMUNICATIONS BIOLOGY, 7 (1). No.-1264. ISSN 2399-3642
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Abstract
The negative membrane potential within bacterial cells is crucial in various essential cellular processes. Sustaining a hyperpolarised membrane could offer a novel strategy to combat antimicrobial resistance. However, it remains uncertain which molecules are responsible for inducing hyperpolarization and what the underlying molecular mechanisms are. Here, we demonstrate that chemically diverse antimicrobial peptides (AMPs) trigger hyperpolarization of the bacterial cytosolic membrane when applied at subinhibitory concentrations. Specifically, these AMPs adopt a membrane-induced amphipathic structure and, thereby, generate hyperpolarization in Escherichia coli without damaging the cell membrane. These AMPs act as selective ionophores for K+ (over Na+) or Cl- (over H2PO4- and NO3-) ions, generating diffusion potential across the membrane. At lower dosages of AMPs, a quasi-steady-state membrane polarisation value is achieved. Our findings highlight the potential of AMPs as a valuable tool for chemically hyperpolarising bacteria, with implications for antimicrobial research and bacterial electrophysiology.
Item Type: | Article |
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Additional Information: | Funding Agency and Grant Number: NKFI [K134754]; National Research Development and Innovation Office 'Elvonal' Programme [KKP 126506]; National Laboratory of Biotechnology [2022-2.1.1-NL-2022-00008]; Janos Bolyai Research Fellowship from the Hungarian Academy of Sciences [BO/608/21]; Ministry of Innovation and Technology of Hungary through the National Research, Development and Innovation Fund [TKP2021-EGA-32]; University of Szeged Open Access Fund [7225] Funding text: T.A.M. acknowledges support from NKFI K134754, C.P. from The National Research Development and Innovation Office 'Elvonal' Programme KKP 126506 and The National Laboratory of Biotechnology Grant 2022-2.1.1-NL-2022-00008, R.S. from The Janos Bolyai Research Fellowship from the Hungarian Academy of Sciences BO/608/21. Support by the Ministry of Innovation and Technology of Hungary through the National Research, Development and Innovation Fund (TKP2021-EGA-32) is acknowledged. University of Szeged Open Access Fund, Grant ID: 7225. |
Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia Q Science / természettudomány > QR Microbiology / mikrobiológia |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 23 Jan 2025 14:54 |
Last Modified: | 23 Jan 2025 14:54 |
URI: | https://real.mtak.hu/id/eprint/214257 |
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