Böde, Kinga and Javornik, Uros and Dlouhy, Ondrej and Zsíros, Ottó and Biswas, Avratanu and Racskóné Domonkos, Ildikó and Sket, Primoz and Karlicky, Vaclav and Ughy, Bettina and Lambrev, Petar and Spunda, Vladimir and Plavec, Janez and Garab, Győző (2024) Role of isotropic lipid phase in the fusion of photosystem II membranes. PHOTOSYNTHESIS RESEARCH, 161. pp. 127-140. ISSN 0166-8595
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Abstract
It has been thoroughly documented, by using P-31-NMR spectroscopy, that plant thylakoid membranes (TMs), in addition to the bilayer (or lamellar, L) phase, contain at least two isotropic (I) lipid phases and an inverted hexagonal (H-II) phase. However, our knowledge concerning the structural and functional roles of the non-bilayer phases is still rudimentary. The objective of the present study is to elucidate the origin of I phases which have been hypothesized to arise, in part, from the fusion of TMs (Garab et al. 2022 Progr Lipid Res 101,163). We take advantage of the selectivity of wheat germ lipase (WGL) in eliminating the I phases of TMs (Dlouh & yacute; et al. 2022 Cells 11: 2681), and the tendency of the so-called BBY particles, stacked photosystem II (PSII) enriched membrane pairs of 300-500 nm in diameter, to form large laterally fused sheets (Dunahay et al. 1984 BBA 764: 179). Our 31P-NMR spectroscopy data show that BBY membranes contain L and I phases. Similar to TMs, WGL selectively eliminated the I phases, which at the same time exerted no effect on the molecular organization and functional activity of PSII membranes. As revealed by sucrose-density centrifugation, magnetic linear dichroism spectroscopy and scanning electron microscopy, WGL disassembled the large laterally fused sheets. These data provide direct experimental evidence on the involvement of I phase(s) in the fusion of stacked PSII membrane pairs, and strongly suggest the role of non-bilayer lipids in the self-assembly of the TM system.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: National Research Development and Innovation Office of Hungary (OTKA K) [128679]; Czech Science Foundation [GACR 23-07744]; European Union under the LERCO project via the Operational Programme Just Transition [CZ.10.03.01/00/22_003/0000003]; HUN-REN Biological Research Centre, Szeged Funding text: This work was supported by grants from the National Research Development and Innovation Office of Hungary (OTKA K 128679 to G.G.), the Czech Science Foundation (GACR 23-07744 S to G.G.) and the European Union under the LERCO project (CZ.10.03.01/00/22_003/0000003) via the Operational Programme Just Transition. CERIC-ERIC provided financial assistance for travel and lodging. Open access funding provided by HUN-REN Biological Research Centre, Szeged. |
| Uncontrolled Keywords: | MODEL; DYNAMICS; HIGHER-PLANTS; Membrane Fusion; CHLOROPHYLL-A; THYLAKOID MEMBRANE; A/B PROTEIN COMPLEX; NONBILAYER LIPIDS; violaxanthin de-epoxidase; linear dichroism spectroscopy; non-bilayer lipids; P-31-NMR spectroscopy; BBY membrane; XANTHOPHYLL CYCLE ENZYME; |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia Q Science / természettudomány > QK Botany / növénytan > QK10 Plant physiology / növényélettan |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 24 Jan 2025 09:55 |
| Last Modified: | 24 Jan 2025 09:55 |
| URI: | https://real.mtak.hu/id/eprint/214308 |
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