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LIP1 Regulates the Plant Circadian Oscillator by Modulating the Function of the Clock Component GIGANTEA

Hajdú, Anita and Nyári, Dóra Vivien and Terecskei, Kata and Gyula, Péter and Ádám, Éva and Dobos, Orsolya and Mérai, Zsuzsanna and Kozma-Bognár, László (2024) LIP1 Regulates the Plant Circadian Oscillator by Modulating the Function of the Clock Component GIGANTEA. CELLS, 13 (17). No.-13171503. ISSN 2073-4409

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Abstract

Circadian clocks are biochemical timers regulating many physiological and molecular processes according to the day/night cycles. The function of the oscillator relies on negative transcriptional/translational feedback loops operated by the so-called clock genes and the encoded clock proteins. Previously, we identified the small GTPase LIGHT INSENSITIVE PERIOD 1 (LIP1) as a circadian-clock-associated protein that regulates light input to the clock in the model plant Arabidopsis thaliana. We showed that LIP1 is also required for suppressing red and blue light-mediated photomorphogenesis, pavement cell shape determination and tolerance to salt stress. Here, we demonstrate that LIP1 is present in a complex of clock proteins GIGANTEA (GI), ZEITLUPE (ZTL) and TIMING OF CAB 1 (TOC1). LIP1 participates in this complex via GUANINE EX-CHANGE FACTOR 7. Analysis of genetic interactions proved that LIP1 affects the oscillator via modulating the function of GI. We show that LIP1 and GI independently and additively regulate photomorphogenesis and salt stress responses, whereas controlling cell shape and photoperiodic flowering are not shared functions of LIP1 and GI. Collectively, our results suggest that LIP1 affects a specific function of GI, possibly by altering binding of GI to downstream signalling components.

Item Type: Article
Additional Information: Funding Agency and Grant Number: NATIONAL RESEARCH, DEVELOPMENT AND INNOVATION OFFICE (Hungary); AUSTRIAN SCIENCE FUND [FWF I3979-B25]; [AN-128740]; [K-134567]; [PD-138963]; [KGYNK-2023] Funding text: This research was funded by NATIONAL RESEARCH, DEVELOPMENT AND INNOVATION OFFICE (Hungary), grant number AN-128740 (L.K.-B.), K-134567 (L.K.-B.) and PD-138963 (A.H.), the HUNGARIAN ACADEMY OF SCIENCES, grant number KGYNK-2023 (A.H.), and the AUSTRIAN SCIENCE FUND, grant number FWF I3979-B25 (Z.M.).
Uncontrolled Keywords: arabidopsis; circadian clock; GIGANTEA; small GTPase LIP1
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
Q Science / természettudomány > QH Natural history / természetrajz > QH426 Genetics / genetika, örökléstan
Q Science / természettudomány > QK Botany / növénytan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 27 Jan 2025 09:09
Last Modified: 27 Jan 2025 09:09
URI: https://real.mtak.hu/id/eprint/214373

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