Hegedűs, Lili and Tóth, Ágnes and Harami, Gábor and Pálinkás, János and Karatayeva, Nargis and Sajben-Nagy, Enikő Ilona and Bene, Szabolcs and Afzali, Sara and Kovács, Mihály and Juhász, Szilvia and Burkovics, Péter (2024) Werner helicase interacting protein 1 contributes to G-quadruplex processing in human cells. SCIENTIFIC REPORTS, 14 (1). No.-15740. ISSN 2045-2322
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Abstract
Genome replication is frequently impeded by highly stable DNA secondary structures, including G-quadruplex (G4) DNA, that can hinder the progression of the replication fork. Human WRNIP1 (Werner helicase Interacting Protein 1) associates with various components of the replication machinery and plays a crucial role in genome maintenance processes. However, its detailed function is still not fully understood. Here we show that human WRNIP1 interacts with G4 structures and provide evidence for its contribution to G4 processing. The absence of WRNIP1 results in elevated levels of G4 structures, DNA damage and chromosome aberrations following treatment with PhenDC3, a G4-stabilizing ligand. Additionally, we establish a functional and physical relationship between WRNIP1 and the PIF1 helicase in G4 processing. In summary, our results suggest that WRNIP1 aids genome replication and maintenance by regulating G4 processing and this activity relies on Pif1 DNA helicase.
Item Type: | Article |
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Additional Information: | Funding Agency and Grant Number: HUN-REN Biological Research Centre, Szeged Funding text: Open access funding provided by HUN-REN Biological Research Centre, Szeged. DAS:All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. |
Uncontrolled Keywords: | WRNIP1, G-quadruplex, PIF1, DNA helicase, Genome stability, Replication |
Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia R Medicine / orvostudomány > RM Therapeutics. Pharmacology / terápia, gyógyszertan |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 27 Jan 2025 09:31 |
Last Modified: | 27 Jan 2025 09:31 |
URI: | https://real.mtak.hu/id/eprint/214377 |
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