Constructing STR Multiplexes for Individual Identification of Hungarian Red Deer

Szabolcsi, Zoltán and Egyed, Balázs and Zenke, Petra and Padar, Zsolt and Borsy, Adrienn Éva and Stéger, Viktor and Csányi, Sándor and Orosz, László (2014) Constructing STR Multiplexes for Individual Identification of Hungarian Red Deer. JOURNAL OF FORENSIC SCIENCES, 59. pp. 1090-1099. ISSN 0022-1198

Text Szabolcsi_et_al_u_135007.538171.pdf Restricted to Repository staff only Download (1MB) | Request a copy

Abstract

Red deer is the most valuable game of the fauna in Hungary, and there is a strong need for genetic identification of individuals. For this purpose, 10 tetranucleotide STR markers were developed and amplified in two 5-plex systems. The study presented here includes the flanking region sequence analysis and the allele nomenclature of the 10 loci as well as the PCR optimization of the DeerPlex I and II. LD pairwise tests and cross-species similarity analyses showed the 10 loci to be independently inherited. Considerable levels of genetic differences between two subpopulations were recorded, and FST was 0.034 using AMOVA. The average probability of identity (PIave) was at the value of 2.6736 × 10−15. This low value for PIave nearly eliminates false identification. An illegal hunting case solved by DeerPlex is described herein. The calculated likelihood ratio (LR) illustrates the potential of the 10 red deer microsatellite markers for forensic investigations.

Actions (login required)

Edit Item