Synthesis and Characterization of Magnetc Nanoparticles for Biological Separation Methods

Ilosvai Ágnes, Ágnes Mária and Szőri-Dorogázi, Emma and Prekob, Ádám and Vanyorek, László (2020) Synthesis and Characterization of Magnetc Nanoparticles for Biological Separation Methods. Materials Science and Engineering, 45 (1). pp. 163-170. ISSN 2063-6792

Preview

Text ISSN_2063_6792_vol_45_1_2020_eng_163-170.pdf Download (459kB) | Preview

Abstract

In our work, we successfully combined sonochemical production with the com-bustion method to produce magnetic nanoparticles. Iron (II)-acetate and iron (III)-citrate pre-cursors were used in the synthesis, which in both cases were dispersed in 400 g/mol polyethy-lene glycol (PEG 400) using a high-efficiency ultrasonic technique (Hielscher homogenizer). The magnetic nanoparticles were bound to DNA to test their applicability in DNA separation processes. Plasmid DNA from Escherichia coli (E. coli) is well suited for carrying out the above assays. The magnetic particle morphology and size distribution were investigated by transmission electron microscopy (TEM), the nanoparticles were characterized by a high de-gree of dispersion. The functional groups on the surface of the particles were identified by Fourier transform infrared spectroscopy (FTIR) and hydroxyl groups were detected. The iron oxide forms of the sample were identified with X-ray diffraction (XRD), which were mag-netite, maghemite and hematite phases. The dispersibility of nanoparticles in aqueous media was characterized by measuring the electrokinetic potential (using DLS) of the particles and due to the hydroxyl groups, its surface was sufficiently dispersible.

Actions (login required)

Edit Item