Incze, Dániel János and Molnár, Zsófia Klára and Nagy, Gergely and Leveles, Ibolya and Vértessy, Beáta (Grolmuszné) and Poppe, László and Bata, Zsófia (2025) Understanding the molecular mechanism of fumonisin esterases by kinetic and structural studies. FOOD CHEMISTRY, 473. No.-143110. ISSN 0308-8146
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Abstract
Fumonisins are sphingolipid-like mycotoxins that cause serious damage by contaminating food and feed. The tricarballylic acid (TCA) units of fumonisin B1 (FB1; accounting for 70 % of fumonisin contamination) can be removed by fumonisin B1 esterase (FE, EC 3.1.1.87) providing a biotechnological FB1 detoxification possibility. Here, we report the regioselective cleavage of the TCA ester at C6 in the first step of FB1 hydrolysis and kinetic characterization for two FEs. The low KM values (4.76–44.3 μM) are comparable to concentrations of environmental contaminations, and the high catalytic efficiencies are promising for practical applications. The X-ray structure of one of the FEs enabled the understanding of the FB1 hydrolysis at molecular level and revealed an arginine pocket key for substrate binding, and the catalytic role of the glutamate preceding the catalytic serine. Computations showed that this FE is likely capable of detoxifying any fumonisin indicating its potential applicability in food and feed products.
| Item Type: | Article |
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| Additional Information: | Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, 1111, Hungary Dr. Bata Ltd., Research and Development Laboratory, Bajcsy-Zsilinszky u. 139, Ócsa, 2364, Hungary Institute of Molecular Life Sciences, HUN-REN, Research Centre for Natural Sciences, Magyar Tudósok krt. 2, Budapest, 1117, Hungary Department of Applied Biotechnology and Food Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, 1111, Hungary Biocatalysis and Biotransformation Research Center, Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University of Cluj-Napoca, Arany János str. 11, Cluj-Napoca, 400028, Romania Export Date: 14 February 2025; Cited By: 0; Correspondence Address: L. Poppe; Budapest University of Technology and Economics, Budapes, Műegyetem rkp. 3, 1111, Hungary; email: poppe.laszlo@vbk.bme.hu; CODEN: FOCHD |
| Uncontrolled Keywords: | Fumonisin esterase, Fumonisin B1, Enzyme mechanism, Acyl migration, Mycotoxin, Protein crystallography |
| Subjects: | Q Science / természettudomány > QR Microbiology / mikrobiológia T Technology / alkalmazott, műszaki tudományok > TP Chemical technology / vegyipar, vegyészeti technológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 04 Sep 2025 08:12 |
| Last Modified: | 04 Sep 2025 08:12 |
| URI: | https://real.mtak.hu/id/eprint/223442 |
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