Bebesi, Tímea and Pálmai, Marcell and Szigyártó, Imola Csilla and Gaál, Anikó and Wacha, András Ferenc and Bóta, Attila and Varga, Zoltán and Mihály, Judith (2025) Surface-enhanced infrared spectroscopic study of extracellular vesicles using plasmonic gold nanoparticles. COLLOIDS AND SURFACES B: BIOINTERFACES, 246. No.-114366. ISSN 0927-7765
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Abstract
Extracellular vesicles (EVs), sub-micrometer lipid-bound particles released by most cells, are considered a novel area in both biology and medicine. Among characterization methods, infrared (IR) spectroscopy, especially attenuated total reflection (ATR), is a rapidly emerging label-free tool for molecular characterization of EVs. The relatively low number of vesicles in biological fluids (~10 10 particle/mL), however, and the complex content of the EVs’ milieu (protein aggregates, lipoproteins, buffer molecules) might result in poor signal-to-noise ratio in the IR analysis of EVs. Exploiting the increment of the electromagnetic field at the surface of plasmonic nanomaterials, surface-enhanced infrared spectroscopy (SEIRS) provides an amplification of characteristic IR signals of EV samples. Negatively charged citrate-capped and positively charged cysteamine-capped gold nanoparticles with around 10 nm diameter were synthesized and tested with blood-derived EVs. Both types of gold nanoparticles contributed to an enhancement of the EVs’ IR spectroscopic signature. Joint evaluation of UV-Vis and IR spectroscopic results, supported by FF-TEM images, revealed that proper interaction of gold nanoparticles with EVs is crucial, and an aggregation or clustering of gold nanoparticles is necessary to obtain the SEIRS effect. Positively charged gold nanoparticles resulted in higher enhancement, probably due to electrostatic interaction with EVs, commonly negatively charged.
| Item Type: | Article |
|---|---|
| Additional Information: | Institute of Materials and Environmental Chemistry, HUN-REN Research Centre for Natural Sciences (RCNS), Magyar tudósok körútja 2, Budapest, 1117, Hungary Hevesy György PhD School of Chemistry, Eötvös Lóránd University, Pázmány Péter sétány 1/A, Budapest, 1117, Hungary Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, 1111, Hungary Department of Chemistry, Eszterházy Károly Catholic University, Leányka u. 6, Eger, 3300, Hungary Export Date: 21 November 2024 CODEN: CSBBE Correspondence Address: Mihály, J.; Institute of Materials and Environmental Chemistry, Magyar tudósok körútja 2, Hungary; email: mihaly.judith@ttk.hu |
| Uncontrolled Keywords: | Extracellular vesicles, Citrate-capped gold nanoparticles, Cysteamine-capped gold nanoparticles, Surface-enhanced infrared spectroscopy (SEIRS), Plasmonics |
| Subjects: | Q Science / természettudomány > QC Physics / fizika > QC173.4 Material science / anyagtudomány Q Science / természettudomány > QD Chemistry / kémia Q Science / természettudomány > QD Chemistry / kémia > QD02 Physical chemistry / fizikai kémia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 23 Sep 2025 13:45 |
| Last Modified: | 23 Sep 2025 13:45 |
| URI: | https://real.mtak.hu/id/eprint/225036 |
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