DNA microsatellite test of thoroughbred and trotter horses in Hungary

Józsa, Csilla and Husvéth, Ferenc and Bán, Beáta and Takács, Erzsébet (2006) DNA microsatellite test of thoroughbred and trotter horses in Hungary. ÁLLATTENYÉSZTÉS ÉS TAKARMÁNYOZÁS, 55 (4). pp. 313-322. ISSN 0230-1814

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Abstract

DNA microsatellite test of Thoroughbred (n=58) and Trotter horses (n=52) was carried out to prove the applicability of this method in the parentage control. The evaluation was made by means of ABI 310 automatic genetic analyser. Among the 12 microsatellites tested the frequence of VHL20, HMS7, ASB2, HTG10 and HMS3 showed strong (P<0,001), while microsatellites AHT4 and HTG7 exhibited somewhat less pronounced (P<0,01) significant differences in gene frequence between Thoroughbred and Trotter horses. Compárising the two breeds, however, no significant differences were detected in the frequency of microsatellites HTG4, AHT5, HTG6 and HMS2. In most cases the distribution of alleles in microsatellites alsó showed significant differences (P<0 001; P<0,01; P<0,05) between the two breeds tested. The results prove that by DNA microsatellites can be used fór the comparison of herds and fór the estimation of genetic distance or similarity.

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