Optimization of seed sterilization and in vitro cultivation methods for effective micropropagation of Arnica montana L.

Kolesnyk, Angela and Kolesnyk, Oleh and Hryshechkina, Olexandra and Kish, Beatris and Mendel, Antoniana and Kryvanych, Olga and Csabai, Judit (2025) Optimization of seed sterilization and in vitro cultivation methods for effective micropropagation of Arnica montana L. In: Fenntartható Tájgazdálkodási Tudományos Műhely konferenciája 2025. Nyíregyházi Egyetem, Műszaki és Agrártudományi Intézet, Nyíregyháza, pp. 6-12. ISBN 9786156032836

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Abstract

Arnica montana is an invaluable medicinal herb, but its wild populations are declining. To aid conservation as well as propagation, the present work investigated methods for sterilizing seeds and culture media for initiating in vitro growth. Seeds sourced in the Ukrainian region surrounding the Krasna mountain range were sterilized by employing different chemicals; sodium hypochlorite gave the most regular and healthy-looking explants. Of the nutrient media tested, Murashige and Skoog (MS) furnished the highest initial development. In IAA-BAP combinations, the best formation was tested under different concentrations. Balanced levels, especially 1.0 mg/L of both growth regulators, resulted in robust as well as regular shooting. While high hormone levels decreased the viability, growth suppression was evident. The results point towards an easy and efficient protocol for starting cultures of A. montana applicable for both research purposes as well as practical cultivation.

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