Mérai, Zsuzsanna and Xu, Fei and Hajdú, Anita and Kozma-Bognár, László and Dolan, Liam (2025) Phytochrome A is required for light-inhibited germination of Aethionema arabicum seed. NEW PHYTOLOGIST, 247 (5). pp. 2134-2146. ISSN 0028-646X
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NewPhytologist-2025-Merai-PhytochromeAisrequiredforlightinhibitedgerminationofAethionemaarabicumseed.pdf Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
The germination of most seeds is influenced by the duration, intensity, and quality of light. The seeds of the model plant Arabidopsis are positive photoblastic and require light to germinate. The germination of negative photoblastic seeds is inhibited by white light. The molecular mechanisms that regulate negative photoblastic germination are unknown due to the lack of a suitable model plant. We identified an accession with negative photoblastic germination in Aethionema arabicum that grows in semi-arid natural habitats. In a forward genetic screen, we identified a mutant - koyash2 (koy2) - that is defective in negative photoblastic germination. There is a nonsense mutation in the gene encoding the phytochrome A photoreceptor in the koy2 mutant. Here, we show that phytochrome A is required for negative photoblastic germination. The defective negative photoblastic phenotype of the koy2 mutant is the result of defective inhibition of germination by the phytochrome A-mediated high irradiance response. This is the first example of phytochrome A-mediated response controlling negative photoblastic seed germination in white, red, far-red, and blue light. We speculate that genetically encoded variation in phytochrome A-mediated germination responses is responsible for local adaptation of Ae. arabicum throughout the Irano-Turanian region.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Austrian Science Fund [I3979-B25/]; National Research, Development and Innovation Office (Hungary) [AN-128740, K-134567, PD-138963]; Austrian Academy of Sciences, and advanced grant from the European Research Council [787613]; Gregor Mendel Institut fur Molekulare Pflanzenbiologie GmbH/KEM Funding text: We thank Eric M. Schranz for providing Aethionema seed stocks. We also thank the staff of the Vienna BioCenter Core Facilities GmbH (VBCF), a member of Vienna BioCenter (VBC), Austria, especially the Plant Sciences Facility for the growth of the plants, the Molecular Biology Unit for providing multiple reagents, the Vienna COVID-19 Detection Initiative (VCDI) for generating a safe work environment during the pandemic and the VBC Child Care Center. We thank Nicole Lettner for the technical support. We thank Ortrun Mittelsten Scheid for the fruitful discussions and advice throughout the project. This research was funded by the Austrian Science Fund (FWF) I3979-B25/ DOI: 10.55776/I3979. For open access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission. It was additionally supported by the National Research, Development and Innovation Office (Hungary), grant nos.: AN-128740 (LKB), K-134567 (LKB) and PD-138963 (AH). LD is funded by the Austrian Academy of Sciences, and advanced grant from the European Research Council (project no.: 787613). Open access funding provided by Gregor Mendel Institut fur Molekulare Pflanzenbiologie GmbH/KEMOE. |
| Uncontrolled Keywords: | INDUCTION; PHOTOINHIBITION; ADAPTATION; ARABIDOPSIS-THALIANA; ACTION SPECTRA; PHYTOCHROME; Light response; Aethionema arabicum; photoblastic phenotype; |
| Subjects: | Q Science / természettudomány > QK Botany / növénytan > QK10 Plant physiology / növényélettan |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 13 Mar 2026 11:53 |
| Last Modified: | 13 Mar 2026 11:53 |
| URI: | https://real.mtak.hu/id/eprint/235679 |
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