Mészáros, Mária and Phan, Thi Ha My and Vigh, Judit Piroska and Porkoláb, Gergő and Kocsis, Anna and Szecskó, Anikó and Páli, Emese Kincső and Cser, Nárcisz Mónika and Polgár, Tamás Ferenc and Kecskeméti, Gábor and Walter, Fruzsina and Schwamborn, Jens C. and Janáky, Tamás and Jan, Jeng-Shiung and Veszelka, Szilvia and Deli, Mária Anna (2025) Alanine and glutathione targeting of dopamine- or ibuprofen-coupled polypeptide nanocarriers increases both crossing and protective effects on a blood–brain barrier model. FLUIDS AND BARRIERS OF THE CNS, 22 (1). No. 18. ISSN 2045-8118
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Abstract
Targeting the blood–brain barrier (BBB) is a key step for effective brain delivery of nanocarriers. We have previously discovered that combinations of BBB nutrient transporter ligands alanine and glutathione (A-GSH), increase the permeability of vesicular and polypeptide nanocarriers containing model cargo across the BBB. Our aim was to investigate dopamine- and ibuprofen-coupled 3-armed poly(<jats:sc>l</jats:sc>-glutamic acid) nanocarriers targeted by A-GSH for transfer across a novel human co-culture model with induced BBB properties. In addition, the protective effect of ibuprofen containing nanoparticles on cytokine-induced barrier damage was also measured.
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| Additional Information: | Funding Agency and Grant Number: HUN-REN Biological Research Centre, Szeged; National Research, Development and Innovation Office of Hungary [NNE-29617, K143766]; National Research, Development and Innovation Office, Budapest, Hungary [PD 138930]; Gedeon Richter Plc. Centenarial Foundation; New National Excellence Program of the Ministry for Innovation and Technology [UNKP-23-3-SZTE-535]; National Academy of Scientist Education Program of the National Biomedical Foundation under the Hungarian Ministry of Culture and Innovation; New National Excellence Program [UNKP-23-3-SZTE-315]; Egyetemi Kutatoi Osztondij Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund [EKOP-393]; Hungarian Research Network [SA-111/2021]; National ScienceTechnology Council, Taiwan [NSTC107-2923-M-006-002-MY3]; Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund [143233] Funding text: Open access funding provided by HUN-REN Biological Research Centre, Szeged. This work was funded by the National Research, Development and Innovation Office of Hungary, grant numbers NNE-29617 (M-ERA.NET2 nanoPD) and K143766 (for M.A.D.). M.M. was supported by the research grant (PD 138930) of the National Research, Development and Innovation Office, Budapest, Hungary, the Gedeon Richter Plc. Centenarial Foundation (H-1103 Budapest, Gyomroi str. 19-21. Hungary). J.P.V. was supported by the New National Excellence Program of the Ministry for Innovation and Technology (UNKP-23-3-SZTE-535). G.P. was supported by the National Academy of Scientist Education Program of the National Biomedical Foundation under the sponsorship of the Hungarian Ministry of Culture and Innovation. E.K.P. was supported by the National Academy of Scientist Education Program of the National Biomedical Foundation under the sponsorship of the Hungarian Ministry of Culture and Innovation. T.F.P. was supported by the UNKP-23-3-SZTE-315 New National Excellence Program and the EKOP-393 Egyetemi Kutatoi Osztondij Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund. F.R.W. was supported by the grant SA-111/2021 from the Hungarian Research Network. J.S.J. was supported by the National ScienceTechnology Council, Taiwan: NSTC107-2923-M-006-002-MY3 (M-ERA.NET2 nanoPD). S.V. was supported by the project no.143233, which has been implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the FK_22 funding scheme. |
| Uncontrolled Keywords: | Blood–brain barrier, In vitro model, Human stem cell derived endothelial cell, Poly(l-glutamic acid), Dualtargeted nanocarriers, Alanine, Glutathione, Dopamine, Ibuprofen |
| Subjects: | R Medicine / orvostudomány > RM Therapeutics. Pharmacology / terápia, gyógyszertan |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 17 Mar 2026 08:08 |
| Last Modified: | 17 Mar 2026 08:08 |
| URI: | https://real.mtak.hu/id/eprint/235753 |
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