Szabó, András and Péter, Mária and Balogh, Gábor and Török, Zsolt and Kóta, Zoltán and Vigh, László and Ali, Omeralfaroug and Timár, Botond and Kövér, György and Nagy, Tibor and Olasz, Ferenc and Bóta, Brigitta and Petneházy, Örs and Gömbös, Patrik and AGYARKO, EDWARD and Nguyen, Anh Thi and Vargáné Visi, Éva and Kovács, Melinda (2025) Liver Lipidomics, Histology, Transcriptomics, and Clinical Chemistry of Rats Intraperitoneally Treated with Fumonisin B1 for 5 days. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 73 (39). pp. 24975-24996. ISSN 0021-8561
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Abstract
Fumonisin B1 was intraperitoneally administered at 2.1 μg/animal/day (1×) and 5 and 10 times above (5× and 10×) to male rats for 5 days (n = 8/group, n = 32). Bodyweight gain decreased in all FB1-treated groups after 3 days, while bodyweight decreased (10×) after 5 days. Feed intake and liver weight decreased (5×, 10×). Clinical chemistry indicated increased total protein, albumin, cholesterol, AST, ALT, and gamma-GT (10×). Histopathology revealed apoptosis, mitosis, hydropic change, mild steatosis, and pericentral glycogen depletion (10×). Shotgun lipidomics showed an increase in sphingosine at 10×, with a dose-dependent depletion of longer-chain ceramide and sphingomyelin molecular species. GM3 ganglioside and free and esterified cholesterol increased linearly with FB1 dose. Arachidonic acid (AA)-containing species of ether-type phosphatidylcholine and phosphatidylethanolamine (PE) displayed a linear dose response; those in diacyl PE and phosphatidylinositol followed logarithmic models. Transcriptomics revealed downregulation of steroid metabolism, while the NF-kB, sphingolipid, PI3K-Akt-PTEN, and TNF signaling pathways were upregulated, critically beyond 5×.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Horizon 2020 Framework Programme Funding text: This work was partially funded by the Hungarian Research Network (HUN-REN-MATE, Mycotoxins in the Food Chain research group) and by the Hungarian National Laboratory project RRF-2.3.1-21-2022-00007, and further by the Flagship Research Groups Programme by the Hungarian University of Agriculture and Life Sciences. HCEMM has received funding from the EU's Horizon 2020 research and innovation program under grant agreement No. 739593 and KIM NKFIA 2022-2.1.1-NL-2022-00005. This research was also funded by the National Research, Development and Innovation Office (2020-1.1.2-PIACI-KFI-2020-00079). The expert help of Krisztina Kiss and Zsolt Egyhazi (Moritz Kaposi Teaching Hospital, Department of Pathology) in histological section preparation is gratefully acknowledged, as is that of Dora Peterfi-Szabo and Tamas Schieszl in the animal handling and Erika Zukic in a lipidomic sample workup. This work is dedicated to the memory of Professor Imre Sarudi. |
| Uncontrolled Keywords: | LIVER; Arachidonic Acid; transcriptomics; LIPIDOMICS; Inflammatory signaling; fumonisin-B1; |
| Subjects: | R Medicine / orvostudomány > RA Public aspects of medicine / orvostudomány társadalmi szerepe > RA0421 Public health. Hygiene. Preventive Medicine / közegészségügy, higiénia, betegség-megelőzés |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 18 Mar 2026 14:44 |
| Last Modified: | 18 Mar 2026 14:44 |
| URI: | https://real.mtak.hu/id/eprint/235835 |
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