Effect of kinetin on lesion development and infection sites in Xanthi-nc tobacco infected by TMV: Single-cell local lesions

Balázs, Ervin and Barna, Balázs and Király, Zoltán (1976) Effect of kinetin on lesion development and infection sites in Xanthi-nc tobacco infected by TMV: Single-cell local lesions. ACTA PHYTOPATHOLOGICA ACADEMIAE SCIENTIARUM HUNGARICAE, 11. pp. 1-9. ISSN 0001-6780

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Abstract

Both the crude and the ultracentrifuged extracts of the kinetin-treated Xanthi-nc tobacco leaf tissues were more infective than was expected on the basis of the number of local lesions caused by TMV. In fact, infectivity of 100 lesions from the kinetintreated half leaves exceeded that of 100 lesions from the control halves. The infective RNA content of the kinetin-treated half leaves did not change in spite of the significant reduction in lesion number. The virus did not become systemic as a result of kinetin treatment. By selective staining of necrotic cells in tissues with Evans blue it was shown that the actual number of local lesions was not reduced as a result of kinetin treatment. This treatment suppresses only the number of necroses visible to the naked eyes, but more necrotic single cells were seen on the kinetin-treated half leaves under the microscope.

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