High incidence of hop stunt Viroid in Hungarian grapevines

Farkas, Erzsébet and Palkovics, László and Mikulás, József and Balázs, Ervin (1999) High incidence of hop stunt Viroid in Hungarian grapevines. ACTA PHYTOPATHOLOGICA ET ENTOMOLOGICA HUNGARICA, 34 (1-2). pp. 7-11. ISSN 0238-1249

Preview

Text Farkas1999_ocr.pdf - Published Version Download (304kB) | Preview

Abstract

The 297 nuclcotide scqucncc of HSVd gvkr, a hop stunt viroid (HSVd gvkr) variam présem in the Hungárián grapcvincs was delcrmincd from cDNAs amplified by the polymerase chain rcaction. HSVd gvkr is very similar in its nuclcotide sequcnccs to scvcral HSVd isolatcs previously delcctcd in cucumbcr, Citrus and grapcvinc. It exhibits 97.6% scqucncc homology with a HSVd grapcvinc isolatc from Gcrmany. HSVd gvkr isolatc sequence was included in the phylogenic trce of different hop stunt viroid sequenccs. Using dót biot detection techniquc an inlcnsivc survcy was performed in different Hungárián vincyards and a high incidence of this viroid was observed. More than 90% of the samples lakén from thrcc independent vincyards were positive fór the HSVd gvkr probe. The wide distribution of this virijíd could be atlributcd to the fact thai grapcvinc nurseries werc nőt screcncd bcforc fór HSVd.

Actions (login required)

Edit Item