Movement-stabilized three-dimensional optical recordings of membrane potential changes and calcium dynamics in hippocampal CA1 dendrites

Gonzalez, Kevin C. and Terada, Satoshi and Noguchi, Asako and Zakka, George N. and O’Toole, Cliodhna and Bilbao, Giuliana and Reynolds, Luke and Jász, Anna and Kertész, Borbála and Szadai, Zoltán and Shen, Alissa and St-Pierre, François and Polleux, Franck and Losonczy, Attila and Rózsa, Balázs (2026) Movement-stabilized three-dimensional optical recordings of membrane potential changes and calcium dynamics in hippocampal CA1 dendrites. NEURON, 114 (10). pp. 1818-1832. ISSN 0896-6273 (print); 1097-4199 (online)

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Abstract

Local dendritic computations are thought to critically influence neuronal signaling and plasticity yet remain largely unexplored in vivo due to challenges in stably imaging small structures at ultrafast timescales. We developed a 3D real-time motion correction platform for movement-stabilized, ultrafast two-photon voltage imaging. By co-labeling CA1 pyramidal neurons with voltage and calcium indicators, we simultaneously measured somato-dendritic and electro-calcium coupling at multiple dendritic sites. We characterized isolated dendritic spikes and distance-dependent backpropagation of naturally occurring and photostimulation-evoked bursts and single spikes. We found that bursts backpropagated more reliably than single spikes, validated that somato-dendritic coupling decreases with distance from soma, and showed that electro-calcium coupling decreases with increasing branch order. These findings provide in vivo evidence for distance-dependent invasion of somatic signals into dendrites, highlight the prevalence of isolated dendritic events, and show that dendritic structure isolates voltage from calcium signaling, potentially enabling unique intracellular pathways in distal dendrites.

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