Potent antibacterial activity from bioactive metabolites of Colletotrichum sp. strain USS33

Tosorn, Piyanoot and Phuchaduek, Supachai and Chukeatirote, Ekachai (2026) Potent antibacterial activity from bioactive metabolites of Colletotrichum sp. strain USS33. ACTA PHYTOPATHOLOGICA ET ENTOMOLOGICA HUNGARICA, 61 (1). pp. 83-94. ISSN 0238-1249

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Abstract

Fungal secondary metabolites are a rich source of bioactive compounds with potential pharmaceutical applications, including antimicrobial agents against multidrug-resistant pathogens. In this study, crude extracts of Colletotrichum sp. strain USS33, cultured in yeast malt broth, were evaluated for antibacterial activity against a panel of Gram-positive and Gram-negative bacteria, including methicillin- and vancomycin-resistant Staphylococcus aureus (MRSA and VRSA). The extracts exhibited broad-spectrum activity, with the strongest effects observed against Pseudomonas fluorescens (23.00 ± 0.23 mm), S . aureus TISTR 1466 (22.00 ± 0.27 mm), and MRSA strain CRP45 (21.50 ± 0.87 mm). Bioautography-guided fractionation revealed that a CH 2 Cl 2 :ethyl acetate (1:1) system effectively separated the active fraction, which retained antibacterial activity against S. aureus , MRSA, and VRSA. GC–MS analysis identified benzeneethanol (11.34%) and long-chain alkenes (1-tetradecene, 1-hexadecene, and 1-octadecene) as major constituents, suggesting that both individual compounds and possible combined effects may contribute to the observed activity. These results highlight Colletotrichum species as a promising source of antibacterial metabolites. Further studies on purification, MIC determination, and mechanism of action are required.

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