Gholizadehfahandari, Fatemeh and Janda, Tibor and Varga, Balázs and György, Márton and Molnár, István and Kruppa, Klaudia Katalin and Türkösi, Edina (2026) Genotype-dependent salt tolerance mechanisms in wheat–Thinopyrum introgression lines revealed by ion transporter gene expression and seedling phenotyping. SCIENTIFIC REPORTS, 16. No. 7647. ISSN 2045-2322
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Abstract
Soil salinity significantly impairs wheat growth and yield worldwide. Wild relatives of wheat, such as Thinopyrum species, harbor valuable salt tolerance traits that can improve cultivated wheat via introgression. This study investigates the salt tolerance of three wheat-Thinopyrum introgression lines and their wheat parents during germination under salt stress (0, 100, 200 mM NaCl). Molecular cytogenetic analyses confirmed the presence and stability of Thinopyrum chromatin in these lines. Morphophysiological traits, including germination rate, radicle and coleoptile length, root system architecture, and biomass were assessed. Salt stress curtailed root and shoot growth across genotypes, though Thinopyrum introgression lines showed varied resilience. Gene expression patterns of key ion transporters (HKT, SOS, NHX) involved in Na+ exclusion and homeostasis were also evaluated in radicle and coleoptile tissues. Results showed significant genotype-dependent differences, with the 3St(3D) substitution line exhibiting strong upregulation of TaSOS1 and TaNHX1 linked to improved salt tolerance mechanisms. Coordinated expression of ion transporter genes suggests enhanced sodium extrusion and sequestration pathways in tolerant genotypes. These findings highlight the potential of wheat-Thinopyrum introgression lines as genetic resources for breeding salt-tolerant wheat cultivars.
| Item Type: | Article |
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| Additional Information: | Department of Plant Physiology and Metabolomics, Agricultural Institute, HUN-REN Centre for Agricultural Research, Martonvásár, 2462, Hungary Department of Cereal Breeding, Agricultural Institute, HUN-REN Centre for Agricultural Research, Martonvásár, 2462, Hungary Department of Biological Resources, Agricultural Institute, HUN-REN Centre for Agricultural Research, Martonvásár, 2462, Hungary Export Date: 10 July 2026; Cited By: 2; Correspondence Address: F. Gholizadeh; Department of Plant Physiology and Metabolomics, Agricultural Institute, HUN-REN Centre for Agricultural Research, Martonvásár, 2462, Hungary; email: fatemeh.gholizadeh@atk.hun-ren.hu; K. Kruppa; Department of Biological Resources, Agricultural Institute, HUN-REN Centre for Agricultural Research, Martonvásár, 2462, Hungary; email: kruppa.klaudia@atk.hun-ren.hu |
| Uncontrolled Keywords: | Salt tolerance, Wheat-Thinopyrum introgression lines, GISH and FISH, Gene expression |
| Subjects: | S Agriculture / mezőgazdaság > S1 Agriculture (General) / mezőgazdaság általában |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 14 Sep 2026 08:11 |
| Last Modified: | 14 Sep 2026 08:11 |
| URI: | https://real.mtak.hu/id/eprint/246113 |
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