Türkösi, Edina and Kruppa, Klaudia Katalin and Darkó, Éva and Varga, Balázs and György, Márton and Gulyás, Zsolt and Jobbágy, Kristóf and Holušová, Kateřina and Gaál, Eszter and Kalapos, Balázs and Károlyiné Cséplő, Mónika and Farkas, András and Ivanizs, László and Mikó, Péter and Gulyás, Andrea and Hidvégi, Norbert Tibor and Kovács, Péter and Cseh, András Attila and Lángné Molnár, Márta and Bartoš, Jan and Szakács, Éva and Molnár, István (2025) Replacement of chromosome 3D with Thinopyrum chromosome 3St led to increased drought tolerance during the flowering stage in wheat. PLANT CELL REPORTS, 44 (11). No. 242. ISSN 0721-7714
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Abstract
The flowering stage is highly susceptible to drought, which significantly reduces wheat grain yield globally. Low genetic diversity in wheat further limits the discovery of optimal gene variants for breeding climate-resilient varieties. The substitution of chromosome 3D by a group 3 chromosome pair from Thinopyrum intermedium × Th. ponticum artificial hybrid was identified using in situ hybridization and genotyping-by-sequencing. This homoeologous substitution showed good functional compensation for grain yield and fertility, similar to the wheat parents ('Mv9kr1' and 'Mv Karizma') in field and greenhouse trials. The substitution line exhibits a semidwarf phenotype due to the Rht8 and Rht2 dwarfing alleles. Automated shoot phenotyping after a 10-day water withdrawal at flowering revealed efficient water preservation allowing to maintain photosynthetic functions, sustained photosynthetic activity, and less chlorophyll degradation, indicated by Normalized Difference Vegetation Index (NDVI) and modified Normalized Difference Index (mND705) values and moderate level of protective functions shown by the expression of stress-related genes. Compared to the wheat parents, the substitution line developed thicker roots with increased volume under drought, resulting in a lower surface-to-volume ratio. This may enhance water storage efficiency and help reduce yield loss under drought conditions.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: HUN-REN Centre for Agricultural Research; Hungarian National Research, Development, and Innovation Office [FK145848, TKP2021-NKTA-06]; EU Horizon Europe project COUSIN [101135314]; MTA Bolyai Janos Research Grant [BO/00384/23/4, BO/00206/24/4, BO/00416/23, BO/00013/25/4]; TowArds Next GENeration Crops [CZ.02.01.01/00/22_008/0004581]; ERDF Programme Johannes Amos Comenius Funding text: Open access funding provided by HUN-REN Centre for Agricultural Research. The research leading to these results received funding from the Hungarian National Research, Development, and Innovation Office under Grant Agreement No FK145848 and TKP2021-NKTA-06 and from the EU Horizon Europe project COUSIN (Nr. 101135314). BV, PM, AC and KK have received funding for their work through the MTA Bolyai Janos Research Grant (BO/00384/23/4, BO/00206/24/4, BO/00416/23 and BO/00013/25/4). JB was supported by the project TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius. |
| Uncontrolled Keywords: | Agropyron glael , In situ hybridization , Genotyping-by-sequencing coverage analysis , Drought tolerance |
| Subjects: | S Agriculture / mezőgazdaság > S1 Agriculture (General) / mezőgazdaság általában |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 14 Sep 2026 11:57 |
| Last Modified: | 14 Sep 2026 11:57 |
| URI: | https://real.mtak.hu/id/eprint/246215 |
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