Cortactin is required for integrin-mediated cell spreading

Illés, András and Enyedi, Balázs and Tamás, Péter and Balázs, Annamária and Bőgel, Gábor and Lukács, Melinda and Buday, László (2006) Cortactin is required for integrin-mediated cell spreading. IMMUNOLOGY LETTERS, 104 (1-2). pp. 124-130. ISSN 0165-2478

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Abstract

Cortactin is an SH3 domain-containing protein that contributes to the formation of dynamic cortical actin-associated structures, such as lamellipodia and membrane ruffles. Here we show that expression of either the GFP-tagged N-terminal or the C-teminal halves of cortactin inhibits significantly the spreading of COS7 cells on fibronectin. Introducing inactivating point mutation into the SH3 domain of the C-terminal half of cortactin suspends the dominant negative effect of the construct. In addition, a vector-based RNA interference was used to knock-down endogenous level of cortactin in cells. We demonstrate that cortactin deficient cells were not able to spread. These results suggest that cortactin is required for integrin-mediated signalling pathways.

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