Imre, Gergely and Juhász, Szilvia (2026) Protocol for cyclic DNA-damaging treatments to generate high mutational burden in the human A549 cell line. STAR PROTOCOLS, 7 (4). No. 104842. ISSN 2666-1667
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Abstract
Here, we present a protocol that describes iterative exposure of the human lung cancer cell line A549 to DNA-damaging agents to progressively increase mutational burden. We describe steps for controlled cytotoxic dosing, metabolic activation, and repeated treatment cycles of up to 70 iterations, followed by clonal isolation. This approach enables the generation of highly mutated cell populations suitable for downstream genomic analyses. For complete details on the use and execution of this protocol, please refer to Juhasz et al.1
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: National Research, Development and Innovation Office, Hungary (NKFIH) [KIM NKFIA TKP-2021-EGA-05, KIM NKFIA 2022-2.1.1-NL-2022-00005]; EU [739593]; H2020-WIDESPREA-01-2016-2017-TeamingPhase2 [GA:739593-HCEMM] Funding text: This work was supported by The National Research, Development and Innovation Office, Hungary (NKFIH) KIM NKFIA TKP-2021-EGA-05 (S.J.) and The National Research, Development and Innovation Office, Hungary (NKFIH) KIM NKFIA 2022-2.1.1-NL-2022-00005 (S.J.). H2020-WIDESPREA-01-2016-2017-TeamingPhase2, GA:739593-HCEMM, EU's Hori-zon 2020 research and innovation program under grant agreement No. 739593(S.J.). |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 05 Oct 2026 14:37 |
| Last Modified: | 05 Oct 2026 14:37 |
| URI: | https://real.mtak.hu/id/eprint/248333 |
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