Xylan-Degrading Catalytic Flagellar Nanorods

Klein, A. and Szabo, V. and Kovacs, M. and Patkó, Dániel and Toth, B. and Vonderviszt, Ferenc (2015) Xylan-Degrading Catalytic Flagellar Nanorods. MOLECULAR BIOTECHNOLOGY, 57 (9). pp. 814-819. ISSN 1073-6085

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Abstract

Flagellin, the main component of flagellar filaments, is a protein possessing polymerization ability. In this work, a novel fusion construct of xylanase A from B. subtilis and Salmonella flagellin was created which is applicable to build xylan-degrading catalytic nanorods of high stability. The FliC-XynA chimera when overexpressed in a flagellin deficient Salmonella host strain was secreted into the culture medium by the flagellum-specific export machinery allowing easy purification. Filamentous assemblies displaying high surface density of catalytic sites were produced by ammonium sulfate-induced polymerization. FliC-XynA nanorods were resistant to proteolytic degradation and preserved their enzymatic activity for a long period of time. Furnishing enzymes with self-assembling ability to build catalytic nanorods offers a promising alternative approach to enzyme immobilization onto nanostructured synthetic scaffolds.

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