CIDRE: an illumination-correction method for optical microscopy

Horváth, Péter and Li, Yunpeng and Csucs, Gabor and Piccinini, Filippo and Smith, Kevin and Balázsi, Csaba (2015) CIDRE: an illumination-correction method for optical microscopy. NATURE METHODS. ISSN 1548-7091

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Abstract

Uneven illumination affects every image acquired by a microscope. It is often overlooked, but it can introduce considerable bias to image measurements. The most reliable correction methods require special reference images, and retrospective alternatives do not fully model the correction process. Our approach overcomes these issues for most optical microscopy applications without the need for reference images.

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