Kárpáti, Éva and Kiss, Péter and Ponyi, Tamás and Fendrik, István and Zamaroczy, Miklós de and Orosz, László (1999) Interaction of Azospirillum lipoferum with wheat germ agglutinin stimulates nitrogen fixation. Journal of Bacteriology, 181 (13). pp. 3949-3955. ISSN 0021-9193 (print), 1098-5530 (online)
|
PDF
1014791.pdf Download (345kB) |
Abstract
In vitro, the nitrogen fixation capability of A. lipoferum is efficiently increased in the presence of wheat germ agglutinin (WGA). A putative WGA-binding receptor, a 32-kDa protein, was detected in the cell capsule. The stimulatory effect required N-acetyl-D-glucosamine dimer (GlcNAc(di)) terminated sugar side chains of the receptor and was dependent on the number of GlcNAc(di) links involved in receptor-WGA interface. Binding to the primary sugar binding sites on WGA had a larger stimulatory effect than binding to the secondary sites. The WGA-receptor complex generated stimulus led to elevated transcription of the nifH and nifA genes and of the glnBA gene cluster but not of the glnA gene from its own promoter. There may well be a signalling cascade contributing to the regulation of nitrogen fixation.
Item Type: | Article |
---|---|
Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH426 Genetics / genetika, örökléstan Q Science / természettudomány > QK Botany / növénytan |
Depositing User: | Erika Bilicsi |
Date Deposited: | 12 Nov 2012 13:37 |
Last Modified: | 12 Nov 2012 13:37 |
URI: | http://real.mtak.hu/id/eprint/3338 |
Actions (login required)
Edit Item |