Specialized Transduction of a Cysteine Marker by Rhizobium meliloti Phage 16-3

Sváb, Zóra and Kondorosi, Ádám and Orosz, László (1978) Specialized Transduction of a Cysteine Marker by Rhizobium meliloti Phage 16-3. Journal of General Microbiology, 106 (2). pp. 321-327. ISSN 0022-1287

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Abstract

Specialized transduction by phage 16-3 of one of the cys markers of Rhizobium meliloti is described. The frequency of transduction was 10−6 to 10−7 per plaque-forming unit. About two-thirds of the transductants were heterogenotes, unstable for the cys+ marker, and yielded high frequency transducing lysates (10−2 per plaque-forming unit). In phage lysates, plaque-forming transducing particles were directly demonstrated while non-plaque-forming particles were only detected indirectly via complementation of defective lysogenic transductants. In the defective transductants, the two distal ends of the vegetative phage map were lacking. By plasmid transfer, the attachment site of phage 16-3 was located close to the cys-46 and met-5 markers. Transduction of the met-5 marker could not be demonstrated.

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