Multiple elements controlling the expression of wheat high molecular weight glutenin paralogs

Makai, Szabolcs and Éva, Csaba and Tamás, László and Juhász, Angéla (2015) Multiple elements controlling the expression of wheat high molecular weight glutenin paralogs. Functional and Integrative Genomics, 15 (6). pp. 661-672. ISSN 1438-793X


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Analysis of gene expression data generated by high-throughput microarray transcript profiling experiments coupled with cis-regulatory elements enrichment study and cluster analysis can be used to define modular gene programs and regulatory networks. Unfortunately, the high molecular weight glutenin subunits of wheat (Triticum aestivum) are more similar than microarray data alone would allow to distinguish between the three homoeologous gene pairs. However, combining complementary DNA (cDNA) expression libraries with microarray data, a co-expressional network was built that highlighted the hidden differences between these highly similar genes. Duplex clusters of cis-regulatory elements were used to focus the co-expressional network of transcription factors to the putative regulatory network of Glu-1 genes. The focused network helped to identify several transcriptional gene programs in the endosperm. Many of these programs demonstrated a conserved temporal pattern across the studied genotypes; however, few others showed variance. Based on this network, transient gene expression assays were performed with mutated promoters to inspect the control of tissue specificity. Results indicated that the interactions of the ABRE│CBF cluster with distal promoter regions may have a dual role in regulation by both recruiting the transcription complex as well as suppressing it in non-endosperm tissue. A putative model of regulation is discussed. © 2015, Springer-Verlag Berlin Heidelberg.

Item Type: Article
Uncontrolled Keywords: regulatory sequence; promoter region; priority journal; plant gene; paralogy; nonhuman; Molecular weight; Glu 1 gene; Genotype; genetic transcription; genetic analysis; gene interaction; Gene Expression; controlled study; ARTICLE; TRANSCRIPTION FACTOR; GLUTENIN; complementary dna; Triticum aestivum; Transient gene assay; tissue specificity; Regulation; PROMOTER; High molecular weight glutenin; Gene program; ENDOSPERM; Co-expression network; cis-regulatory module
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
Q Science / természettudomány > QK Botany / növénytan > QK10 Plant physiology / növényélettan
S Agriculture / mezőgazdaság > SB Plant culture / növénytermesztés
Depositing User: MTMT SWORD
Date Deposited: 26 Apr 2016 07:06
Last Modified: 02 May 2016 11:41

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