İdil, Önder and Macit, İkbal and Kaygusuz, Özge and Darcan, Cihan (2016) The role of oxidative stress genes and effect of pH on methylene blue sensitized photooxidation of Escherichia coli. Acta Biologica Hungarica, 67 (1). pp. 85-98. ISSN 0236-5383
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Abstract
In this study, the survival time of wild type E. coli W3110 and 11 mutants was analysed with a plate count method in methylene blue added or control groups under daylight fluoroscence illumination (4950 lux) at different pH values (5.0, 6.0, 7.0, and 8.0) in phosphate buffer. As a result, while the number of bacteria did not decrease under photooxidative stress at pH 5.0 and 6.0 during a 6-hour incubation, the wild type and all mutants decreased more than 2 log. at pH 8.0, and approximately one log. at pH 7.0. It was determined that a 2 log decrease in wild type E. coli takes 3.7 h according to t<sub>99</sub> value at pH 8, these values were 2.39 h in the katE mutant, 2.64 h in the soxR mutant, 2.67 h in the <bold>oxyR</bold> mutant, 2.71 h in the sodB mutant, 3 h in the btuE mutant, 3.38 h in the zwf mutant and 3.40 h in the soxS mutant, respectively (p < 0.05). The roles of these genes were proved with complement tests. Finally, it is found that the effectiveness of photooxidative stress is in direct relation with pH, and the katE, soxR, oxyR, sodB, btuE, zwf, and soxS genes are important for the protection against this stress.
Item Type: | Article |
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Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia |
Depositing User: | Ágnes Sallai |
Date Deposited: | 08 Jun 2016 08:15 |
Last Modified: | 31 Mar 2017 23:15 |
URI: | http://real.mtak.hu/id/eprint/35636 |
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