Characterization of a New Wheat- 1M(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit

Zhou, J. P. and Cheng, Y. and Zang, L. L. and Yang, E. N. and Liu, C. and Zheng, X. L. and Deng, K. J. and Zhu, Y. Q. and Zhang, Y. (2016) Characterization of a New Wheat- 1M(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit. Cereal Research Communications, 44 (2). pp. 198-205. ISSN 0133-3720

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Abstract

In this study, a new substitution line, 12-5-1, with 42 chromosomes that was derived from BC₃F₂ descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. The 12-5-1 was immune to both powdery mildew and stripe rust and has stable fertility. Multi-color fluorescence in situ hybridization indicated that 12-5-1 was a substitution line 1M^b(1B). The seed storage protein electrophoresis showed that 12-5-1 presented high molecular weight glutenin subunits (2 + 12) of CN19 and a new subunit designated as M which apparently originated from parent Ae. biuncialis, and absent 7 + 8 subunits. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness and mixing time of 12-5-1 were signifiantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1M^b-specifi polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1026, TNAC1041, TNAC1-02 and TNAC1-04, were also obtained. The new substitution line 1M^b(1B) 12-5-1 could be a valuable source for wheat improvement, especially for wheat end product quality and resistance to disease.

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