Interactions of the carrier ligands of antidiabetic metal complexes with human serum albumin: a combined spectroscopic and separation approach with molecular modeling studies

Enyedy, Éva Anna and Horváth, László and Hetényi, Anasztázia and Tuccinardi, Tiziano and Hartinger, Christian G. and Kiss, Tamás (2011) Interactions of the carrier ligands of antidiabetic metal complexes with human serum albumin: a combined spectroscopic and separation approach with molecular modeling studies. BIOORGANIC & MEDICINAL CHEMISTRY, 19 (14). pp. 4202-4210. ISSN 0968-0896

Text 1_s2.0_S0968089611004275_main_u.pdf Restricted to Registered users only Download (999kB) | Request a copy

Abstract

The specific binding of carrier ligands of antidiabetic vanadium(IV) and zinc(II) complexes into drug binding pockets of human serum albumin (HSA) has been investigated via displacement reactions of site markers such as warfarin and dansylglycine by different spectroscopic (fluorescence, circular dichroism, NMR) and separation methods (capillary zone electrophoresis, ultrafiltration-UV). Conditional stability constants of the ligands were calculated for the binding at sites I and II of HSA. Binding site I was found to be the primary binding site for 2,6-pyridine dicarboxylic acid (dipic) and picolinic acid (pic), and site II for 6-methylpicolinic acid (6-Mepic) and maltol, although dipic, 6-Mepic and pic displace both site markers at differing extents. The experimental data is complemented by protein–ligand docking calculations for dipic and 6-Mepic which support the observations.

Actions (login required)

Edit Item